Genetic analysis of oligodendrocyte specification project

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A short lead paragraph

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Objective

Our goal is to identify genes essential for neural development by screening for mutations that disrupt formation of the nervous system. In particular, we screen transgenic zebrafish that express fluorescent proteins in central and peripheral glial cells, looking for mutations that alter the number, distribution and differentiation of myelinating glia. We are using next-generation sequencing and bioinformatics to accelerate the process of moving from mutant phenotype identification to mutant gene identification.

Major results

In our first year of screening, we have identified about 20 different mutant lines of zebrafish. For our first gene identification project, we chose to use RNA-Seq to obtain gene expression data in wild-type and mutant embryos and, at the same time, attempt to identify the causative mutation. We are currently analyzing potential mutations in several candidate genes.

Project lead

Bruce Appel

Users

Funding

Links to award summaries for each source of funding
  • Genetic Analysis of Oligodendrocyte Specification NIH/NINDS NS046668

Recent publications

Journal articles

Conferences

External links

Links to project's websites

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